Cell Line



BCRC Number: 60283
Name: BxPC-3
Type: human pancreatic adenocarcinoma
Species: Homo sapiens (human)
Age Stage: 61 years
Gender: unknown
Tissue: pancreas; adenocarcinoma

Morphology: epithelial
Split Ratio: 1:3 to 1:6
Isoenzyme Analysis: NP, G6PD, MD, LD
Mycoplasma Test: negative
Cytogenetics: near triploid
Tumorigenic: yes, in nude mice

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: the cells do not express the cystic fibrosis transmembrane condctance regulator (CFTR)
Others: STR-PCR profile — D7S820:10, 13 CSF1PO:13 TH01:9 D13S317:11 D16S539:9, 11 vWA:14, 18 TPOX:8 Amelogenin:X D5S818:11
Product Character: mucin; pancreas cancer specific antigen (pancreas cancer associated antigen); carcinoembryonic antigen (CEA)

Image / Description:   200倍視野下
  100倍視野下 高細胞密度
  200倍視野下 高細胞密度
 

Biosafety Level: 1
Reference: Cancer Invest. 4: 15-23, 1986; Clin. Lab. Med. 2: 567-578, 1982.
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