Cell Line



BCRC Number: 60279
Name: MG-63
Type: human osteogenic sarcoma, interferon producer
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 14 years
Gender: unknown
Tissue: bone; osteosarcoma

Morphology: fibroblast
Split Ratio: 1:4 to 1:8
Isoenzyme Analysis: NP, G6PD, MD, LD
Mycoplasma Test: negative
Cytogenetics: hypotriploid; modal chromosome number is 66
Receptor expressed: transforming growth factor beta (TGF-beta) RI, transforming growth factor beta (TGF-beta) RII

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% heat-inactivated fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: high level of interferon production can be induced using polyinosinic acid-polycytidilic acid, cycloheximide and actinomycin D
Others: STR-PCR profile — D7S820:10 CSF1PO:10, 12 TH01:9.3 D13S317:11 D16S539:11 vWA:16, 19 TPOX:8, 11 Amelogenin:X, Y D5S818:11, 12
Product Character: interferon

Image / Description:   No Description.
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Biosafety Level: 1
Reference: Antimicrob. Agents Chemother. 12: 11-15, 1977.
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