Cell Line



BCRC Number: 60278 Add To Bookmark
Name: Indian muntjac
Type: muntjac skin
Species: Muntiacus muntjak (muntjac)
Age Stage: adult
Gender: unknown
Tissue: skin

Morphology: fibroblast
Split Ratio: 1:2 to 1:4
Isoenzyme Analysis: NP, G6PD, MD, LD
Mycoplasma Test: negative
Virus resistance: poliovirus 1
Virus susceptibility: vesicular stomatitis (Indiana); herpes simplex; vaccinia

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 80% Ham's F10 medium + 20% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: established from a skin biopsy from an adult male muntjac; muntjacs, members of a primitive subfamily of the deer family, Cervidae, have a diploid chromosome number of 7 in the male and 6 in the female (Science 168: 1364-1366, 1970); this is the lowest number yet described in a mammal and should be most valuable for mammalian genetic studies as well as investigations relating to chromosomal evolution; recent tests for bovine viral diarrhea virus (BVDV) indicate that the line is producing both detectable BVDV antigens and infectious BVDV virions (In Virol Methods 48: 211-221, 1994) .

Image / Description:   200倍視野下
 

Biosafety Level: 2
Reference: Science 168: 1364-1366, 1970
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