Cell Line



BCRC Number: 60274 Add To Bookmark
Name: RPMI 7951
Type: Human malignant melanoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 18 years
Gender: Female
Tissue: melanoma; malignant; skin; metastatic site: lymph node

Morphology: Epithelial
Split Ratio: 1:2 to 1:4
Mycoplasma Test: Negative; NOTE: A contaminant identified as Mycoplasma fermentans was eliminated in 1975.
Karyology: Hyperdiploid; modal number = 49
Tumorigenic: yes, in nude mice; forms pigmented melanoma

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium (Eagle) in Earle's BSS with 0.1mM non-essential amino acids and 1 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Others: STR-PCR profile — D7S820:11, 12 CSF1PO:12 TH01:9, 9.3 D13S317:11, 12 D16S539:11, 12 vWA:17, 19 TPOX:8 Amelogenin:X D5S818:11

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 59: 301-307, 1977
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