Cell Line



BCRC Number: 60271 Add To Bookmark
Name: OKM 1
Type: mouse hybridoma, anti human monocytes
Species: Mus musculus (mouse )
Strain Ethnicity: BALB/c (B cell); BALB/c (myeloma)
Gender: unknown
Tissue: Hybridoma; B lymphocyte

Morphology: Lymphoblast
Split Ratio: maintain cell density between 105 to 106 cells/ml
Mycoplasma Test: Negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 80% Iscove's modified Dulbecco's medium + 20% fetal bovine serum
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 1-2 x 105 cells ml
Medium Renewal: every 2 to 3 days

Description: Formed by the fusion of P3X63Ag8U1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood mononuclear cells; the cells produce monoclonal antibody (IgG2b) reactive to human granulocytes, monocytes and null cells
Product Character: immunoglobulin; monoclonal antibody; against human granulocyte; against CD11b; cross-reacts with human mononuclear cells (monocytes); isotype: IgG2b

Image / Description:   200倍視野下
 

Biosafety Level: 1
Patent Statement: U.S. Pat. 4,364,936
Reference: Proc. Natl. Acad. Sci. USA 77: 4914-4917, 1980; U.S. Pat. 4,364,936 dated Dec. 21, 1982
Shipping/Field/Price:
Frozen Vial for all NTD$ 7000 Order this item

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