Cell Line



BCRC Number: 60268
Name: Hs 695T
Type: Human amelanotic melanoma, metastatic to lymph node
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 26 years
Gender: Male
Tissue: melanoma; amelanotic; skin; from metastatic site: lymph node

Morphology: Epithelial
Split Ratio: 1:2 to 1:8
Mycoplasma Test: Negative
Karyology: Hyperdiploid; modal number = 52
Tumorigenic: yes, in immunosuppressed mice

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Established from the lymph node metastasis of an amelanotic melanoma; grow moderately in semisolid medium; not tumorigenic in antithymocyte-serum-treated mice
Others: STR-PCR profile — D7S820:9, 10 CSF1PO:11 TH01:6 D13S317:12 D16S539:9, 13 vWA:18 TPOX:8 Amelogenin:X, Y D5S818:9

Image / Description:   100倍視野下
 

Biosafety Level: 1
Reference: In Vitro 15: 342-350, 1979
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