Cell Line



BCRC Number: 60263 Add To Bookmark
Name: A375.S2
Type: Human malignant melanoma
Species: Homo sapiens (human)
Age Stage: 54 years
Gender: Female
Tissue: melanoma; malignant; skin

Morphology: Epithelial
Split Ratio: 1:3 to 1:8
Mycoplasma Test: Negative
Tumorigenic: yes, in immunosuppressed mice

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Cloned from the human malignant melanoma A375 cell line (see BCRC 60039) by limiting dilution; the cells will eventually lyse and die in the presence of IL-1 and therefore can be used in a reliable and sensitive growth inhibition assay for IL-1.
Others: STR-PCR profile — D7S820:9 CSF1PO:11, 12 TH01:8 D13S317:11, 14 D16S539:9 vWA:16, 17 TPOX:8, 10 Amelogenin:X D5S818:12

Image / Description:   100倍視野下
  100倍視野下
  200倍視野下
  100倍視野下 高細胞密度
  200倍視野下 高細胞密度
 

Biosafety Level: 1
Reference: J. Immunol. 135: 3962-3968, 1985; J. Immunol. 136: 3098-3102, 1986; Biochem. Biophys. Res. Commun. 154: 1189-1196, 1988; J. Natl. Cancer Inst. 51: 1417-1423, 1973
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