Cell Line



BCRC Number: 60252
Name: Ramos (RA 1)
Type: Human American Burkitt's lymphoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 3 years
Gender: Male
Tissue: lymphoma; Burkitt's lymphoma (American); B lymphocyte

Morphology: Lymphoblast
Split Ratio: maintain cell density between 105 to 106 cells/ml
Mycoplasma Test: Negative
Karyology: Hypodiploid; modal number = 45
Tumorigenic: yes, forms tumors in nude mice
Receptor expressed: interleukin 4 (IL-4); low affinity IgE (CD23)

Incubation Temp(°C): 37°C
Culture Medium: 90% RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate + 10% heat-inactivated fetal bovine serum
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 1-2 x 105 cells ml
Medium Renewal: every 2 to 3 days

Others: STR-PCR profile — D7S820:11 CSF1PO:10, 11 TH01:7, 9.3 D13S317:13, 14 D16S539:10, 13 vWA:15, 16 TPOX:8, 9 Amelogenin:X D5S818:7, 12
Product Character: immunoglobulin (surface and secreted)

Image / Description:   100倍視野下
 

Biosafety Level: 1
Reference: Intervirology 5: 319-334, 1975; Int. J. Cancer 19: 337-344, 1977; J. Immunol. 129: 1336-1342, 1982; J. Immunol. 140: 2625-2632, 1988
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