Cell Line

BCRC Number: 60251
Name: Ca Ski
Type: Human cervical epidermoid carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 40 years
Gender: Female
Tissue: Cervix; epidermoid carcinoma; from metastatic site: cervix

Morphology: Epithelial
Split Ratio: 1:3 to 1:8
Mycoplasma Test: Negative
Karyology: Hypertriploid

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RMPI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose , 10 mM HEPES, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Established from epidermoid carcinoma of the cervix metastatic to the small bowel mesentery of a 40-year-old Caucasian; the cells secrete the beta subunit of human chorionic gonadotropin (beta-hCG) and express tumor-associated antigen; the cells are reported to contain an integrated human papillomavirus type 16 genome (HPV-16, about 600 copies per cell) as well as sequences related to HPV-18.
Others: STR-PCR profile — D7S820:8, 11 CSF1PO:10 TH01:7 D13S317:8, 12 D16S539:11, 12 vWA:17 TPOX:8 Amelogenin:X D5S818:13
Product Character: beta subunit of human chorionic gonadotropin (hCG); tumor associated antigen

Image / Description:   100倍視野下低細胞密度

Biosafety Level: 2
Reference: Science 196: 1456-1458, 1977; J. Virol. 61: 962-971, 1987; Virology 145: 313-318, 1985; Am. J. Pathol. 119: 361-366, 1985
Frozen Vial for all NTD$ 6000 Order this item

Bioresource Collection and Research Center

331, Shih-Pin Rd., Hsinchu 30062, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

Copyright © 2018.BCRC All rights reserved.

web maintainance: +886-3-5223191 ext 593