Cell Line



BCRC Number: 60250
Name: T-47D
Type: human breast ductal carcinoma
Species: Homo sapiens (human)
Age Stage: 54 years
Gender: unknown
Tissue: breast; mammary gland; ductal carcinoma; pleural effusion

Morphology: epithelial
Split Ratio: 1:3 to 1:5
Isoenzyme Analysis: NP, G6PD, MD, LD
Mycoplasma Test: negative
Cytogenetics: hypotriploid; modal chromosome number=65
Doubling time: 32 hours
Receptor expressed: calcitonin; androgen receptor, positive; progesterone receptor, positive; glucocorticoid; prolactin; estrogen receptor, positive

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate + 10% fetal bovine serum, supplemented with 0.2 I.U. bovine insulin per ml.
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Others: STR-PCR profile — D7S820:11 CSF1PO:11, 13 TH01:6 D13S317:12 D16S539:10 vWA:14 TPOX:11 Amelogenin:X D5S818:12

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: Biochem. Biophys. Res. Commun. 101: 1131-1138, 1981; Biochem. J. 200: 315-320, 1981; J. Biol. Chem. 256: 12269-12274, 1981
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