Cell Line



BCRC Number: 60249
Name: SW480
Type: Human colon adenocarcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 50 years; Dukes' type B
Gender: Male
Tissue: Colon; adenocarcinoma

Morphology: Epithelial
Split Ratio: 1:2 to 1:8
Mycoplasma Test: Negative
Karyology: Hypotriploid
Virus susceptibility: human immunodeficiency virus (HIV, LAV)
Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
Receptor expressed: epidermal growth factor (EGF)

Incubation Temp(°C): 37°C
Incubation Condition: without CO2 in air
Culture Medium: 90% Leibovitz's L-15 medium with 2 mM L-glutamine + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: established from a primary adenocarcinoma of the colon; a cell line established from a lymph node metastasis taken from the same patient one year later is available (SW620, see ATCC CCL-227 or CCRC 60343); the cell line is negative for CSAp (CSAp-) and colon antigen 3.The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes.
Others: STR-PCR profile — D7S820:8 CSF1PO:13, 14 TH01:8 D13S317:12 D16S539:13 vWA:16 TPOX:11 Amelogenin:X D5S818:13
Product Character: carcinoembryonic antigen (CEA) 0.7 ng/10 exp6 cells/10 days; keratin; transforming growth factor beta

Image / Description:   200倍視野下
  100倍視野下
  400倍視野下
 

Biosafety Level: 1
Reference: Cancer Res. 36: 4562-4569, 1976; J. Natl. Cancer Inst. 59: 221-226, 1977; J. Virol. 61: 209-213, 1987; Cancer 76: 201-209, 1995; Int. J. Cancer 41: 287-296, 1988; Int. J. Cancer 54: 693-699, 1993; Cancer Res. 54: 3700-3702, 1994; Proc. Natl. Acad. Sci. US
Shipping/Field/Price:
Frozen Vial for all NTD$ 6000 Order this item

331, Shih-Pin Rd., Hsinchu 30062, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

web maintainance: +886-3-5223191 ext 593

Copyright © 2018.BCRC All rights reserved.The duplication or use of information and data such as texts or images or any linkage the website at the "bcrc.firdi.org.tw" is only permitted with the indication of the source or with prior approval by the BCRC(Bioresource Collection and Research Center).