Cell Line



BCRC Number: 60249
Name: SW480
Type: Human colon adenocarcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 50 years; Dukes' type B
Gender: Male
Tissue: Colon; adenocarcinoma

Morphology: Epithelial
Split Ratio: 1:2 to 1:8
Mycoplasma Test: Negative
Karyology: Hypotriploid
Virus susceptibility: human immunodeficiency virus (HIV, LAV)
Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
Receptor expressed: epidermal growth factor (EGF)

Incubation Temp(°C): 37°C
Incubation Condition: without CO2 in air
Culture Medium: 90% Leibovitz's L-15 medium with 2 mM L-glutamine + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: established from a primary adenocarcinoma of the colon; a cell line established from a lymph node metastasis taken from the same patient one year later is available (SW620, see ATCC CCL-227 or CCRC 60343); the cell line is negative for CSAp (CSAp-) and colon antigen 3.The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes.
Others: STR-PCR profile — D7S820:8 CSF1PO:13, 14 TH01:8 D13S317:12 D16S539:13 vWA:16 TPOX:11 Amelogenin:X D5S818:13
Product Character: carcinoembryonic antigen (CEA) 0.7 ng/10 exp6 cells/10 days; keratin; transforming growth factor beta

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Biosafety Level: 1
Reference: Cancer Res. 36: 4562-4569, 1976; J. Natl. Cancer Inst. 59: 221-226, 1977; J. Virol. 61: 209-213, 1987; Cancer 76: 201-209, 1995; Int. J. Cancer 41: 287-296, 1988; Int. J. Cancer 54: 693-699, 1993; Cancer Res. 54: 3700-3702, 1994; Proc. Natl. Acad. Sci. US
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