Cell Line



BCRC Number: 60246 Add To Bookmark
Name: ZF4
Type: Zebrafish embryo
Species: Brachydanio rerio (zebrafish)
Age Stage: Embryo
Gender: unknown
Tissue: Embryo

Morphology: fibroblast
Split Ratio: 1:2 to 1:4
Mycoplasma Test: Negative
Cytogenetics: hyperploid; range = 110 to 120
Karyology: Hyperploid; range = 110 to 120

Incubation Temp(°C): 28°C
Incubation Condition: 5% CO2
Culture Medium: 90% A 1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: by 0.25% trypsin solution; NOTE: DO NOT USE EDTA TO DISSOCIATE THE CELLS.
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Established from one-day-old zebrafish embryos; the cells are cultivated at 28°C and can be used as a transfection host; the cells can be killed within 10 days by culturing in medium containing 0.8 to 1.0 mg ml G418

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Biosafety Level: 1
Reference: In Vitro Cell. Dev. Biol. Anim. 29A: 749-754, 1993
Shipping/Field/Price:
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