Cell Line



BCRC Number: 60244
Name: AtT-20
Type: Mouse pituitary tumor, adrenocorticotrophin secreting
Species: Mus musculus (mouse)
Strain Ethnicity: LAF1
Gender: unknown
Tissue: Pituitary; tumor

Morphology: small round cell clusters
Split Ratio: 1:2
Mycoplasma Test: Negative
Karyology: Normal; diploid
Virus resistance: poliovirus 1
Virus susceptibility: vesicular stomatitis

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 82.5% F-12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate + 15% horse serum + 2.5% fetal bovine serum
Growth Property: Suspension; the cells grow as floating clusters of cells; the clusters are viable
SubCulture Procedure: Allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. Proliferation is enhanced by incubation on a shaking platform; NOTE: The line grows as floating clusters of cells. This is essential for propagation of the line. Care should be taken when subculturing to insure the retention of aggregates.
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: at time of subculture

Description: this is an ACTH secreting line
Product Character: adrenocorticotropic hormone (ACTH)

Biosafety Level: 1
Reference: Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962; Proc. Soc. Exp. Biol. Med. 84: 253-254, 1953
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