Cell Line



BCRC Number: 60243
Name: 786-O
Type: Human primary renal cell adenocarcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 58 years
Gender: Male
Tissue: Kidney; renal cell adenocarcinoma

Morphology: Epithelial
Split Ratio: 1:4 to 1:12
Mycoplasma Test: Negative
Cytogenetics: hypertriploid
Karyology: Hypertriploid
Tumorigenic: yes, in immunosuppressed hamsters

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Others: STR-PCR profile — D7S820:11, 12 CSF1PO:10 TH01:6, 9.3 D13S317:8 D16S539:12 vWA:15, 17 TPOX:8, 11 Amelogenin:X, Y D5S818:9
Product Character: parathyroid hormone (PTH) like peptide

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Biosafety Level: 1
Reference: In Vitro 12: 623-627, 1976; In Vitro 14: 779-786, 1978; Proc. Natl. Acad. Sci. USA 85: 4605-4609, 1988
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