Cell Line



BCRC Number: 60241 Add To Bookmark
Name: A-498
Type: Human kidney carcinoma
Species: Homo sapiens (human)
Age Stage: 52 years
Gender: Female
Tissue: Kidney; carcinoma

Morphology: Epithelial
Split Ratio: 1:2 to 1:4
Mycoplasma Test: Negative
Karyology: Hypertriploid; modal number = 74
Tumorigenic: yes; in nude mice; forms undifferentiated carcinoma; also forms tumors in anti thymocyte serum treated newborn mice

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Others: STR-PCR profile — D7S820:10, 11 CSF1PO:11, 12 TH01:6, 9.3 D13S317:12 D16S539:12 vWA:18 TPOX:8, 11 Amelogenin:X D5S818:11, 13

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 51: 1417-1423, 1973
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