Cell Line

BCRC Number: 60240
Name: A2058
Type: Human metastatic melanoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 43 years
Gender: Male
Tissue: melanoma; skin; from metastatic site: lymph node

Morphology: Epithelial
Split Ratio: 1:6 to 1:12
Mycoplasma Test: Negative
Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
Receptor expressed: nerve growth factor (NGF); laminin

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 1 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Established from a lymph node metastasis removed from a 43-year-old male Caucasian with malignant melanoma; the cells are highly invasive and provide a source of cellular invasion associated proteins (such as the 72000 dalton type IV collagenase).Tissue inhibitor of metalloproteinase-2 TIMP-2, autocrine motility factor and the 67000 dalton laminin receptor.
Others: STR-PCR profile — D7S820:11 CSF1PO:10, 11 TH01:7, 9 D13S317:13, 14 D16S539:9, 13 vWA:14, 18 TPOX:8 Amelogenin:X, Y D5S818:9, 12

Image / Description:   200倍視野下

Biosafety Level: 1
Patent Statement: U.S. and/or other patent application
Reference: Proc. Natl. Acad. Sci. USA 74: 565-569, 1977; Proc. Natl. Acad. Sci. USA 76: 1288-1292, 1979; Proc. Natl. Acad. Sci. USA 77: 5258-5262, 1980
Frozen Vial for all NTD$ 6000 Order this item

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