Cell Line



BCRC Number: 60238 Add To Bookmark
Name: MEG-01
Type: Human megakaryoblastic leukemia
Species: Homo sapiens (human)
Age Stage: 55 years
Gender: Male
Tissue: leukemia; chronic myelogenous leukemia (CML); megakaryoblast

Morphology: lymphoblast
Split Ratio: 1:2 to 1:3
Mycoplasma Test: Negative
Cytogenetics: hyperdiploid; modal number = 56 to 58; the Philadelphia chromosome (Ph1) is present
Karyology: Hyperdiploid; modal number is 56 to 58; the Philadelphia chromosome (Ph1) is present
Doubling time: 36 to 48 hrs

Incubation Temp(°C): 37°C
Culture Medium: 90 % RMPI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose , 10 mM HEPES, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: mixed attached and suspension
SubCulture Procedure: Scrape the adherent cells into the medium, and dilute the resulting suspension into fresh medium in new flasks
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from bone marrow cells taken from a 55-year-old male with blast crisis of Philadelphia (Ph1) chromosome-positive chronic myelogenous leukemia (CML); the cells are positive for cytoplasmic Factor VIII and surface GPIIb/IIIa, periodic acid - Schiff(PAS) reaction, alpha naphthyl acetate esterase and acid phosphatase; are negative for myeloperoxidase, alpha naphthyl butyrate esterase, naphthol AS-D chloroacetate esterase and alkaline phosphatase.
Others: STR-PCR profile — D7S820:11 CSF1PO:10 TH01:7 D13S317:8 D16S539:9 vWA:16 TPOX:8, 11 Amelogenin:X, Y D5S818:13

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Biosafety Level: 1
Reference: Blood 66: 1384-1392, 1985
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