Cell Line



BCRC Number: 60235 Add To Bookmark
Name: OKT 4
Type: mouse hybridoma, anti human inducer/helper T lymphocyte
Species: Mus musculus (B cell); Mus musculus (myeloma) (mouse (B cell); mouse (myeloma))
Strain Ethnicity: CAF-1; BALB/c (myeloma)
Gender: unknown
Tissue: Hybridoma; B lymphocyte

Morphology: Lymphoblast
Split Ratio: maintain cell density between 105 to 106 cells/ml
Mycoplasma Test: Negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 80% Iscove's modified Dulbecco's medium + 20% fetal bovine serum
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: Start cultures at 2 x 10^5 viable cells/ml and maintain cell density between 1 x 10^5 and 1 x 10^6 viable cells/ml.
Medium Renewal: every 2 to 3 days

Description: Formed by fusing P3X63Ag8.U1 mouse myeloma cells with mouse spleen cells immunized with human peripheral blood T lymphocyte; the cells produce monoclonal antibody (IgG2b) reactive to human helper/inducer T lymphocytes.
Product Character: immunoglobulin; monoclonal antibody; against human T cell (human helper/inducer T lymphocyte); against CD4; isotype: IgG2b

Image / Description:   100倍視野下
  200倍視野下
 

Biosafety Level: 1
Patent Statement: U.S. Patent No. 4,381,295
Reference: Proc. Natl. Acad. Sci. USA 77: 4914-4917, 1980; U.S. Pat. 4,381,295 dated Apr. 26, 1983
Shipping/Field/Price:
Frozen Vial for all NTD$ 7000 Order this item

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