Cell Line



BCRC Number: 60223
Name: PLC/PRF/5 (Alexander)
Type: Human hepatoma
Species: Homo sapiens (human)
Gender: unknown
Tissue: Liver; hepatoma; Alexander cells

Morphology: Epithelial
Split Ratio: 1:2 to 1:4
Mycoplasma Test: Negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% fetal bovine serum,
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Others: STR-PCR profile — D7S820:9, 11 CSF1PO:10 TH01:8 D13S317:11, 12 D16S539:13 vWA:15, 16 TPOX:8 Amelogenin:X D5S818:12
Product Character: hepatitis virus B surface antigen (HBsAg)

Image / Description:   200倍視野下
 

Biosafety Level: 2
Patent Statement: U.S. and or other Patent application
Reference: Br. J. Cancer 34: 509-515, 1976; Nature 282: 617-618, 1979; Science 209: 407, 1980; S. Afr. J. Med. Sci. 41: 8-9, 1976; Exp. Cell. Res. 200: 235-241, 1992; S. Afr. Med. J. 50: 2124-2128, 1976
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