Cell Line



BCRC Number: 60218 Add To Bookmark
Name: BRL 3A
Type: Buffalo rat liver
Species: Rattus norvegicus (rat)
Strain Ethnicity: Buffalo
Gender: unknown
Tissue: Liver

Morphology: Epithelial
Split Ratio: 1:3 to 1:6
Mycoplasma Test: Negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 95% Modified F-12K + 5% fetal bovine serum (Pro. Natl. Acad. Sci. USA 62: 852-859, 1969)--OR--Modified Eagle's medium with 10 or 20% tryptose phosphate broth (Cell 11: 441-446, 1977)--OR--Minimum essential medium (Eagle) with 2 mM L-glutamine and Hank's B
Growth Property: Adherent
SubCulture Procedure: CTC (collagenase, trypsin, and chick serum) in Dulbecco's phosphate buffer saline without calcium and magnesium; Trypsin-EDTA could also be used
Freezed Medium: 85% culture medium + 10% fetal bovine serum + 5% DMSO
Medium Renewal: every 2 to 3 days

Description: the line was originally isolated by primary cloning; the serum-free conditioned medium from this cell line is a source of MSA; MSA is a family of polypeptides that can partially satisfy the serum requirement of chick embryo fibroblasts; for 24 hrs after initiating culture from thawed ampule or after subcultureing,after 24hrs, the cells may be transferred to serum free medium.
Product Character: somatomedin like multiplication stimulating activity (MSA)

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: Pro. Natl. Acad. Sci. USA 62: 852-859, 1969; Carnegie Inst. Yearbook 67: 419-421, 1968; J. Cell Physiol. 81: 153-170, 1973; J. Cell Physiol. 90: 127-138, 1977; Cell 11: 441-446, 1977
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