Cell Line



BCRC Number: 60217 Add To Bookmark
Name: NCI-N87
Type: Human gastric carcinoma
Species: Homo sapiens (human)
Gender: Male
Tissue: Stomach; gastric carcinoma; from metastatic site: liver

Morphology: Epithelial
Split Ratio: 1:3 to 1:4
Mycoplasma Test: Negative
Karyology: Near diploid; modal number = 43; DM were found in 64% of the cells
Tumorigenic: yes; the cells form tumors in athymic nude mice
Doubling time: 47hr
Receptor expressed: acetylcholine, muscarinic

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: derived from a liver metastasis of a well differentiated carcinoma of the stomach taken prior to cytotoxic therapy; the tumor was passaged as a xenograft in athymic nude mice for three passages before the cell line was established; the cells express carcinoembryonic antigen (CEA) and TAG 72, and are L-dopa decarboxylase (DDC) negative. They were minimally positive for vasoactive intestinal peptide (VIP) receptors, lacked gastrin receptors, and expressed receptors for muscarinic cholinergic agents. No evidence of amplification or rearrangements was noted with the N-myc, L-myc, myb and EGF receptor genes. The cell line expressed levels of c-myc and c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide. NCI-N87 cells have a reported plating efficiency of 4.3%.
Others: STR-PCR profile — D7S820:10, 11 CSF1PO:8, 12 TH01:9 D13S317:8, 11 D16S539:9, 13 vWA:15, 16 TPOX:9, 11 Amelogenin:X, Y D5S818:12, 13

Image / Description:   No Description.
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  high density
  high density
 

Biosafety Level: 1
Reference: Cancer Res. 50: 2773-2780, 1990
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