Cell Line

BCRC Number: 60213
Name: SNU-5
Type: Human gastric carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Asian (Korean)
Age Stage: 33 years
Gender: Female
Tissue: stomach; gastric carcinoma; metastatic site: ascites

Morphology: suspension, multicell aggregates
Mycoplasma Test: Negative
Karyology: Near tetraploid; modal number = 89; DM were found
Receptor expressed: vasoactive intestinal peptide (VIP); acetylcholine, muscarinic

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 80% Iscove's modified Dulbecco's medium + 20% fetal bovine serum
Growth Property: Clusters in suspension
SubCulture Procedure: Addition or replacement of fresh medium; add medium as the cell density increases
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from ascites of a patient with poorly differentiated carcinoma of the stomach; the patient had previously received chemotherapy including 5-fluorouracil, doxorubicin, and mitomycin C; the cells express the surface carcinoembryonic antigen (CEA) and TAG-72.There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.

Image / Description:   200視野下

Biosafety Level: 1
Reference: Cancer Res. 50: 2773-2780, 1990
Frozen Vial for all NTD$ 6000 Order this item

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