Cell Line



BCRC Number: 60212
Name: SNU-16
Type: Human gastric carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Asian (Korean)
Age Stage: 33 years
Gender: Female
Tissue: Stomach; carcinoma

Morphology: Round cell clusters, epithelial
Mycoplasma Test: Negative
Karyology: Near tetraploid; modal number = 92; DM were found; four HSR were present
Doubling time: 27hrs
Receptor expressed: vasoactive intestinal peptide (VIP); acetylcholine, muscarinic

Incubation Temp(°C): 37°C
Culture Medium: 90% RPMI 1640 medium + 10% fetal bovine serum
Growth Property: Clusters in suspension
SubCulture Procedure: Addition or replacement of fresh medium; add medium as the cell density increases
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from ascites of a patient with poorly differentiated carcinoma of the stomach; the line was established from cells taken prior to chemotherapy; the cells express the surface carcinoembryonic antigen (CEA) and TAG-72; the cells are L-dopa decarboxylase (DDC) positive.
Others: STR-PCR profile — D7S820:12 CSF1PO:12 TH01:6, 9 D13S317:8, 12 D16S539:11, 13 vWA:16 TPOX:11 Amelogenin:X D5S818:10, 13

Image / Description:   200視野下
 

Biosafety Level: 1
Reference: Cancer Res. 50: 2773-2780, 1990
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