Cell Line

BCRC Number: 60210
Name: SNU-1
Type: Human gastric carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Asian (Korean)
Age Stage: 44 years
Gender: Male
Tissue: Stomach; carcinoma

Morphology: round cell clusters partial attached cells are epithelial
Mycoplasma Test: Negative
Karyology: Hyperdiploid; modal number = 47; DM were found
Receptor expressed: vasoactive intestinal peptide (VIP)

Incubation Temp(°C): 37°C
Culture Medium: 90% RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Clusters in suspension
SubCulture Procedure: Addition or replacement of fresh medium; add medium as the cell density increases
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from a poorly differentiated primary carcinoma of the stomach from a 44-year-old Korean male which was taken prior to cytotoxic therapy; the cells express and secrete carcinoembryonic antigen (CEA) and TAG-72 (900 ng/10(6) cells); The cell line expressed levels of c-myc and c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.
Others: STR-PCR profile — D7S820:9,12 CSF1PO:12,13 TH01:8,9.3 D13S317:11,14 D16S539:9,12 vWA:14 TPOX:8,10 Amelogenin:X,Y D5S818:12,13

Image / Description:   200視野下

Biosafety Level: 1
Reference: Cancer Res. 50: 2773-2780, 1990
Frozen Vial for all NTD$ 6000 Order this item

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