Cell Line



BCRC Number: 60209 Add To Bookmark
Name: H-4-II-E
Type: Rat hepatoma
Species: Rattus norvegicus (rat)
Gender: unknown
Tissue: Liver; hepatoma

Morphology: Epithelial
Split Ratio: 1:2 to 1:4
Mycoplasma Test: Negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 80% Minimum essential medium Eagle with 0.1 mM non-essential amino acids and Earle's BSS + 10% fetal bovine serum + 10% calf serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: the line, which has been cured of Mycoplasma arginini contamination, still exhibits high aryl hydrocarbon hydroxylase inducibility; it is useful for detecting picogram quantities of planar polychlorinated organic compounds in environmental samples and food extracts.
Product Character: aryl hydrocarbon hydroxylase is inducible

Image / Description:   200視野下
 

Biosafety Level: 1
Reference: In Vitro 16: 230, 1980; Natl. Cancer Inst. Monogr. 13: 229-245, 1964; Cell Tissue Kinet. 10: 233-243, 1977; J. Assoc. Off. Anal. Chem. 62: 904-916, 1979; Biochem. Pharmacol. 22: 2766-2769, 1973
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