Cell Line



BCRC Number: 60208 Add To Bookmark
Name: tk- ts13
Type: Syrian hamster kidney, thymidine kinase, temperature-sensitive mutant
Species: Mesocricetus auratus (hamster, Syrian golden)
Gender: unknown
Tissue: Kidney

Morphology: Epithelial
Mycoplasma Test: Negative

Incubation Temp(°C): 34°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 2 x 104 cells/sq. cm.
Medium Renewal: every 2 to 3 days

Description: the line is a derivative of BHK-21; the cells are temperature sensitive for growth, and are thymidine kinase deficient (TK-); the line is an efficient host for transfection studies; the cells should be grown at 34°C

Image / Description:   200視野下
 

Biosafety Level: 1
Reference: J. Cell Physiol. 92: 425-436, 1977; Mol. Cell Biol. 2: 1145-1154, 1982
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