Cell Line



BCRC Number: 60205
Name: ES-D3
Type: mouse pluripotent embryonic stem cell
Species: Mus musculus (mouse)
Strain Ethnicity: 129/Sv+c/+p
Age Stage: embryo; blastocyst
Gender: unknown
Tissue: embryo; pluripotent embryonic stem cell

Morphology: spherical
Split Ratio: 1:10 to 1:20
Mycoplasma Test: negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 85% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM 2-mercaptoethanol + 15% fetal bovine serum (1,000 U/ml or 10 ng/ml of LIF (optional))
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA NOTE: To maintain the cells in an undifferentiated state they must be grown with irradiated or mitomycin C treated mouse fibroblasts.
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: The clonal embryonic stem cell line ES-D3 was derived from a strain of 129/Sv+C/+P mouse blastocyst; The cells spontaneously differentiate into embryonic structures in the absence of a feeder layer or conditioned medium. They can be maintained in the undifferentiated state by frequent subculture (every 2 to 3 days) on confluent feeder layers (STO cells) irradiated at 12,000 Rads.

Image / Description:   200視野下
 

Biosafety Level: 1
Reference: J. Embryol. Exp. Morphol. 87: 27-45, 1985; Nature 336: 684-687, 1988; Proc. Natl. Acad. Sci. USA 83: 9065-9069, 1986; Proc. Natl. Acad. Sci. USA 85: 8583-8587, 1988
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