Cell Line



BCRC Number: 60204
Name: IMR-90
Type: human fetal lung
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 16 weeks gestation
Gender: unknown
Tissue: lung; normal; fibroblast

Morphology: fibroblast
Split Ratio: 1:2 to 1:8
Isoenzyme Analysis: NP, G6PD, MD, LD
Mycoplasma Test: negative
Cytogenetics: diploid; normal human female; stable
Virus susceptibility: poliovirus 1,2,3; herpes simplex 1,2; varicella zoster; vesicular stomatitis; cytomegalovirus; vaccinia

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 3 to 4 days

Description: derived from the lungs of a 16 week female fetus; the division potential, viral susceptibilities and other properties have been thoroughly studied such that the line may be considered as an alternate for WI-38 and other standard human lung cell strains.
Others: STR-PCR profile — D7S820:9, 12 CSF1PO:11, 13 TH01:8, 9.3 D13S317:11, 13 D16S539:10, 13 vWA:16, 19 TPOX:8, 9 Amelogenin:X D5S818:12, 13

Image / Description:   200視野下
  100倍視野下
  200倍視野下
 

Biosafety Level: 1
Reference: Science 196: 60-63, 1977
Shipping/Field/Price:
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