Cell Line



BCRC Number: 60200
Name: KATO III
Type: human gastric carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Asian
Age Stage: 55 years
Gender: unknown
Tissue: stomach; gastric carcinoma; pleural effusion; metastatic site: supraclavicular and axillary lymph nodes and douglas' cul-de-sac

Morphology: spherical
Split Ratio: 1:2 to 1:8
Isoenzyme Analysis: NP, G6PD, MD, LD
Mycoplasma Test: negative; NOTE: The line has been cured of a previous mycoplasma infection at ATCC.
Cytogenetics: hypotetraploid
Tumorigenic: yes, in cheek pouches of anti thymocyte serum treated hamsters; not tumorigenic in nude mice

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 80% Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate + 20% fetal bovine serum. (RPMI 1640 may be substituted for the Iscove medium)
Growth Property: suspension
SubCulture Procedure: addition or replacement of fresh medium; adherent cells can be dislodged by scraping
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Others: STR-PCR profile — D7S820:8,12 CSF1PO:7,11 TH01:7,9 D13S317:8,12 D16S539:10,12 vWA:14, 16 TPOX:11 Amelogenin:X D5S818:10,11

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: Japan. J. Exp. Med. 48: 61-68, 1978
Shipping/Field/Price:
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