Cell Line



BCRC Number: 60198
Name: RBL-1
Type: Rat basophilic leukemia
Species: Rattus norvegicus (rat)
Strain Ethnicity: Wistar
Gender: unknown
Tissue: leukemia; basophil; chemically induced

Morphology: Lymphoblast
Split Ratio: maintain cell density between 105 to 106 cells/ml
Mycoplasma Test: Negative
Receptor expressed: FcERI (Fc of IgE)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 80-90% Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate + 10-20% fetal bovine serum
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 3 x 105 cells/ml
Medium Renewal: every 2 to 3 days

Description: the line exhibits various characteristics of basophil differentiation including histamine release and surface receptors for IgE
Product Character: histamine

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: Nat. New Biol. 244: 73-76, 1973; J. Exp. Med. 139: 600-616, 1974
Shipping/Field/Price:
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