Cell Line



BCRC Number: 60192
Name: Daudi
Type: Human Burkitt's lymphoma
Species: Homo sapiens (human)
Strain Ethnicity: Black
Age Stage: 16 years
Gender: Male
Tissue: Peripheral blood, Burkitt's lymphoma; B lymphoblast

Morphology: Lymphoblast
Split Ratio: maintain cell density between 3-5x10(5) to2-3 x 10(6)cells/ml
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Cytogenetics: diploid; normal male
Karyology: Normal male; diploid; stable
Tumorigenic: yes, in nude mice; form colonies in agarose
Receptor expressed: complement; Fc

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 3-5 x 105 cells ml
Medium Renewal: every 2 to 3 days

Description: Derived from a 16-year-old Black male with Burkitt's lymphoma; the cells are positive for EBNA(only EBNA1 and EBNA3-6, Journal of Cell Science 110, 2579-2587,1997) and VCA and exhibit surface markers for the Fc fragment of IgG, complement receptors and surface bound immunoglobulin; the line carries Epstein-Barr virus.It is a well characterized B lymphoblast cell line which has been employed in studies of mechanisms of leukemogenesis.
Others: STR-PCR profile — D7S820:8, 10 CSF1PO:12 TH01:6, 7 D13S317:11, 12 D16S539:10, 12 vWA:15, 17 TPOX:8, 11 Amelogenin:X, Y D5S818:8, 13

Image / Description:   200倍視野下
 

Biosafety Level: 2
Reference: Cancer Res. 28: 1300-1310, 1968; Clin. Exp. Immunol. 25: 367-378, 1976; Int. J. Cancer 19: 337-344, 1977
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