Cell Line



BCRC Number: 60192
Name: Daudi
Type: Human Burkitt's lymphoma
Species: Homo sapiens (human)
Strain Ethnicity: Black
Age Stage: 16 years
Gender: Male
Tissue: Peripheral blood, Burkitt's lymphoma; B lymphoblast

Morphology: Lymphoblast
Split Ratio: maintain cell density between 3-5x10(5) to2-3 x 10(6)cells/ml
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Cytogenetics: diploid; normal male
Karyology: Normal male; diploid; stable
Tumorigenic: yes, in nude mice; form colonies in agarose
Receptor expressed: complement; Fc

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 3-5 x 105 cells ml
Medium Renewal: every 2 to 3 days

Description: Derived from a 16-year-old Black male with Burkitt's lymphoma; the cells are positive for EBNA(only EBNA1 and EBNA3-6, Journal of Cell Science 110, 2579-2587,1997) and VCA and exhibit surface markers for the Fc fragment of IgG, complement receptors and surface bound immunoglobulin; the line carries Epstein-Barr virus.It is a well characterized B lymphoblast cell line which has been employed in studies of mechanisms of leukemogenesis.
Others: STR-PCR profile — D7S820:8, 10 CSF1PO:12 TH01:6, 7 D13S317:11, 12 D16S539:10, 12 vWA:15, 17 TPOX:8, 11 Amelogenin:X, Y D5S818:8, 13

Image / Description:   200倍視野下
 

Biosafety Level: 2
Reference: Cancer Res. 28: 1300-1310, 1968; Clin. Exp. Immunol. 25: 367-378, 1976; Int. J. Cancer 19: 337-344, 1977
Shipping/Field/Price:
Frozen Vial for all NTD$ 6000 Order this item

331, Shih-Pin Rd., Hsinchu 30062, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

web maintainance: +886-3-5223191 ext 593

Copyright © 2018.BCRC All rights reserved.The duplication or use of information and data such as texts or images or any linkage the website at the "bcrc.firdi.org.tw" is only permitted with the indication of the source or with prior approval by the BCRC(Bioresource Collection and Research Center).