Cell Line



BCRC Number: 60191
Name: SUP-T1
Type: Human T cell lymphoblastic lymphoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 8 years
Gender: Male
Tissue: leukemia; lymphoblastic; T lymphoblast

Morphology: Lymphoblast
Split Ratio: maintain cell density between 2x10(5) to 2x10(6) cells/ml
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Karyology: 92, XXYY; abnormal mosaic male karyotype; chromosomal translocations involve both the alpha and beta T cell receptor.
Virus susceptibility: Human immunodeficiency virus 1 (HIV-1)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2 in 100% humidity
Culture Medium: 90% RPMI 1640 medium + 10% fetal bovine serum
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 2-5 x 105 cells ml
Medium Renewal: every 2 to 3 days

Description: Derived from a malignant pleural effusion from a 8-year-old Caucasian male with T cell lymphoblastic lymphoma (T-LL); the cells are E-rosette, Leu 5, OKT9 and CALLA negative; the cells express the beta 2M, Leu 1, Leu 2, Leu 3a, Leu 4, Leu6, Leu 9, and OKT 10 antigens.
Others: STR-PCR profile — D7S820:11 CSF1PO:10,11 TH01:9.3 D13S317:11,12 D16S539:9,11,12 vWA:16, 18 TPOX:9 Amelogenin:X,Y D5S818:11,12
Product Character: immunoglobulin (cytoplasmic)

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: Cancer Res. 44: 5657-5660, 1984; Cell 43: 705-713, 1985; Cell 50: 107-117, 1987; Science 226: 1445-4117, 1984; Science 232: 1123-1127, 1986; Blood 71: 395-402, 1988
Shipping/Field/Price:
Frozen Vial for all NTD$ 6000 Order this item

331, Shih-Pin Rd., Hsinchu 30062, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

web maintainance: +886-3-5223191 ext 593

Copyright © 2018.BCRC All rights reserved.The duplication or use of information and data such as texts or images or any linkage the website at the "bcrc.firdi.org.tw" is only permitted with the indication of the source or with prior approval by the BCRC(Bioresource Collection and Research Center).