Cell Line

BCRC Number: 60191
Name: SUP-T1
Type: Human T cell lymphoblastic lymphoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 8 years
Gender: Male
Tissue: leukemia; lymphoblastic; T lymphoblast

Morphology: Lymphoblast
Split Ratio: maintain cell density between 2x10(5) to 2x10(6) cells/ml
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Karyology: 92, XXYY; abnormal mosaic male karyotype; chromosomal translocations involve both the alpha and beta T cell receptor.
Virus susceptibility: Human immunodeficiency virus 1 (HIV-1)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2 in 100% humidity
Culture Medium: 90% RPMI 1640 medium + 10% fetal bovine serum
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 2-5 x 105 cells ml
Medium Renewal: every 2 to 3 days

Description: Derived from a malignant pleural effusion from a 8-year-old Caucasian male with T cell lymphoblastic lymphoma (T-LL); the cells are E-rosette, Leu 5, OKT9 and CALLA negative; the cells express the beta 2M, Leu 1, Leu 2, Leu 3a, Leu 4, Leu6, Leu 9, and OKT 10 antigens.
Others: STR-PCR profile — D7S820:11 CSF1PO:10,11 TH01:9.3 D13S317:11,12 D16S539:9,11,12 vWA:16, 18 TPOX:9 Amelogenin:X,Y D5S818:11,12
Product Character: immunoglobulin (cytoplasmic)

Image / Description:   200倍視野下

Biosafety Level: 1
Reference: Cancer Res. 44: 5657-5660, 1984; Cell 43: 705-713, 1985; Cell 50: 107-117, 1987; Science 226: 1445-4117, 1984; Science 232: 1123-1127, 1986; Blood 71: 395-402, 1988
Frozen Vial for all NTD$ 6000 Order this item

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