Cell Line

BCRC Number: 60189 Add To Bookmark
Name: RA3-3A1/6.1
Type: Rat x mouse hybridoma, anti mouse B cell surface glycoprotein B220
Species: Mus musculus (mouse )
Strain Ethnicity: Lewis (B cell); BALB/c (myeloma)
Gender: unknown
Tissue: Hybridoma; B lymphocyte

Morphology: Lymphoblast
Split Ratio: Don't allow the cell concentration to exceed 5x10(5) cells/ml
Mycoplasma Test: Negative
Receptor expressed: immunoglobulin; monoclonal antibody; against the B220 mouse B cell antigen (200000 dalton form of the T200 glycoprotein, CD45R); isotype: IgM

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4.5 g/L glucose + 10% fetal bovine serum
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium; Do not allow the cell concentration to exceed 5 x 105 cells/ml
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 3-4 x 104 cells ml
Medium Renewal: every 2 to 3 days

Description: Formed by the fusion of spleen cells from a Lewis rat immunized with the mouse cell line RAW 112, a pre-B cell tumor, with the mouse myeloma S194/5.XXO.BU1; the line secretes a monoclonal rat IgM antibody specific for the 200,000 MW form of T200 glycoprot

Image / Description:   200倍視野下

Biosafety Level: 1
Reference: Nature 289: 681-683, 1981
Frozen Vial for all NTD$ 6000 Order this item

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