Cell Line



BCRC Number: 60187
Name: U-2 OS
Type: Human osterogenic sarcoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 15 years
Gender: Female
Tissue: Bone; osteosarcoma

Morphology: epithelial
Split Ratio: 1:3 to 1:6
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative; NOTE: The line has been cured of a previous mycoplasma infection at ATCC.
Karyology: Hypertriploid with highly altered chromosomes
Receptor expressed: insulin-like growth factor I (IGF-I); insulin-like growth factor II (IGF II)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% McCoy's 5a medium with 1.5 mM L-glutamine + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Others: STR-PCR profile — D7S820:11, 12 CSF1PO:13 TH01:6, 9.3 D13S317:13 D16S539:11, 12 vWA:14, 18 TPOX:11, 12 Amelogenin:X D5S818:11
Product Character: osteosacoma derived growth factor (ODGF)

Image / Description:   200倍視野下
  100倍視野下高密度細胞照片
  200倍視野下高密度細胞照片
 

Biosafety Level: 1
Reference: Int. J. Cancer 2: 434-447, 1967; Nature 319: 511-514, 1986; J. Cell. Physiol. 159: 531-541, 1994
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