Cell Line



BCRC Number: 60184 Add To Bookmark
Name: 2254-62.2
Type: Baby hamster kidney, derivative of BHK-21 (C-13)
Species: Hamster
Gender: unknown
Tissue: Kidney

Morphology: Fibroblast
Split Ratio: 1:3 to 1:8
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 93% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 100 nM methotrexate + 7% dialyzed fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: This line was derived from BHK-21(C-13)(see BCRC 60041); this line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs (e.g., Factor VIII)

Image / Description:   100倍視野下
 

Biosafety Level: 1
Patent Statement: U.S. Pat. 4,965,199
Reference: U.S. Pat. 4,965,199 dated Oct. 23, 1990
Shipping/Field/Price:
Frozen Vial for all NTD$ 6000 Order this item

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