Cell Line



BCRC Number: 60183 Add To Bookmark
Name: V79-4
Type: Chinese hamster lung
Species: Cricetulus griseus (hamster, Chinese)
Gender: Male
Tissue: Lung

Morphology: Epithelial
Split Ratio: 1:6 to 1:14
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Cytogenetics: Pseudodiploid; modal number = 22; range = 20 to 23
Karyology: Pseudodiploid; modal number = 22

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate 4.5 g/L glucose and 1.0mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: This cell line was developed by Ford and Yerganian in 1958 from lung tissue of a young male Chinese hamster, and was originally designated Strain V; the line was renamed V79 by Elkind in 1958,and was used in studies of X-ray induced damage and repair processes; in 1966, E.H.Y. Chu obtained the line from W. Sinclair, and isolated the V79-4 clone; the cells have a high plating efficiency (80%), and a generation time of 12 to 14 hours

Image / Description:   No Description.
  No Description.
 

Biosafety Level: 1
Reference: Molecular cell genetics. New York: Wiley; 1985:pp. 66-74; Genetics 62: 359-377, 1969; Proc. Natl. Acad. Sci. USA 61: 1306-1312, 1968; Nature 184: 1293-1295, 1959; J. Natl. Cancer Inst. 21: 393-425, 1958; J. Virol. 70:6323-6335,1996
Shipping/Field/Price:
Frozen Vial for all NTD$ 7000 Order this item

Bioresource Collection and Research Center

331, Shih-Pin Rd., Hsinchu 300193, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

Copyright © 2018.BCRC All rights reserved.

web maintenance: +886-3-5223191 ext 572

Privacy Policy        Cookie