Cell Line

BCRC Number: 60180 Add To Bookmark
Name: BNL CL.2
Type: Mouse BALB/c embryonic liver
Species: Mus musculus (mouse)
Strain Ethnicity: BALB/c
Age Stage: Embryo
Gender: unknown
Tissue: Liver; normal

Morphology: Epithelial
Split Ratio: 1:2 to 1:6
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Tumorigenic: no; The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose,90%;fetal bovine serum,10%.
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: this is a normal embryonic liver cell line derived from a BALB/c mouse; it was selected to grow in medium in which ornithine was substituted for arginine and phenylalanine for tyrosine; these cells do not form colonies in 0.3% agarose or produce tumors in either normal syngeneic or immunodeficient ATXFL mice.

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Biosafety Level: 1
Reference: Nature 276:510-511, 1978
Frozen Vial for all NTD$ 6000 Order this item

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