Cell Line



BCRC Number: 60179 Add To Bookmark
Name: EL4
Type: Mouse lymphoma, benzo(a)pyrene induced leukemia
Species: Mus musculus (mouse)
Strain Ethnicity: C57BL/6N
Gender: unknown
Tissue: Lymphoma; T lymphocyte

Morphology: Lymphoblast
Split Ratio: maintain cell density between 105 to 106 cells/ml
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Karyology: Hyperdiploid; modal number = 39

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose,90%;horse serum,10%.
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 1-2 x 105 cells ml
Medium Renewal: every 2 to 3 days

Description: Established from a lymphoma induced in a C57BL mouse by 9,10-dimethyl-1,2-benzanthracene; the cells express G antigen (a surface antigen induced by leukemia type G (Gross) virus), H-2b and Thy-1.2; the cells do not bear TL (thymus leukemia) antigen or surface immunoglobulin; the line is resistant to 0.1 mM cortisol and dexamethasone but sensitive to 20 ug/ml PHA

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: Br. J. Cancer 4: 372-379, 1950; J. Natl. Cancer Inst. 48:265-271, 1972; J. Natl. Cancer Inst. 51:883-890, 1973; Cancer Res. 25: 813-819, 1965; J. Immunol. 110: 1470-1475, 1973; J. Immunol. 108:1146-1151, 1972
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