Cell Line

BCRC Number: 60178 Add To Bookmark
Name: P815
Type: Mouse mastocytoma
Species: Mus musculus (mouse)
Strain Ethnicity: DBA/2
Gender: unknown
Tissue: Mast cell; mastocytoma

Split Ratio: maintain cell density between 105 to 106 cells/ml
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose,90%;fetal bovine serum,10%.
Growth Property: Suspension (some adherent cells)
SubCulture Procedure: Addition or replacement of fresh medium; Adherent cells can be recovered by scraping.
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 1-2 x 105 cells/ml
Medium Renewal: every 2 to 3 days

Description: the cells phagocytose latex beads but not zymosan or BCG; they do not function in antibody dependent cell mediated cytotoxicity; growth of the cells is not inhibited by dextran sulfate, LPS or PPD
Product Character: lysozyme

Image / Description:   100X視野下

Biosafety Level: 1
Reference: Biochem. Biophys. Res. Commun. 61: 1268-1275, 1974; Cell. Immunol. 9: 60-66, 1973; J. Immunol. 119: 950-954, 1977; J. Immunol. 111: 389-394, 1973; Cancer Res. 37: 546-550, 1977; Nature 249: 49-51, 1974; J. Exp. Med. 143: 1528-1533, 1976
Frozen Vial for all NTD$ 6000 Order this item

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