Cell Line



BCRC Number: 60177
Name: C3A (HepG2/C3A)
Type: Human hepatoblastoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 15 years
Gender: Male
Tissue: liver; hepatoblastoma

Morphology: Epithelial
Split Ratio: 1:4 to 1:6
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Receptor expressed: insulin; insulin-like growth factor II (IGF II)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate,90%;fetal bovine serum,10%.
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: The line is clonal derivative of Hep G2 that was selected for strong contact inhibition of growth, high albumin production, high production of alpha fetoprotein (AFP) and ability to grow in glucose deficient medium; as the cells become confluent, there is a marked reduction in AFP secretion and an increase in albumin secretion; gluconeogenesis activity is strongly oxygen dependent; the cells have nitrogen metabolizing activity comparable to perfused rat livers; there is no evidence of a Hepatitis B virus genome in this cell line.
Others: STR-PCR profile — D7S820:10 CSF1PO:10, 11 TH01:9 D13S317:9, 13 D16S539:12, 13 vWA:17 TPOX:8, 9 Amelogenin:X, Y D5S818:11, 13
Product Character: 'The line is clonal derivative of Hep G2 that was selected for strong contact inhibition of growth, high albumin production, high production of alpha fetoprotein (AFP) and ability to grow in glucose deficient medium; as the cells become confluent, there is a marked reduction in AFP secretion and an increase in albumin secretion; gluconeogenesis activity is strongly oxygen dependent; the cells have nitrogen metabolizing activity comparable to perfused rat livers; there is no evidence of a Hepatitis B virus genome in this cell line

Image / Description:   100倍視野下(低密度)
  200倍視野下(低密度)
  100倍視野下(高密度)
  200倍視野下(高密度)
 

Biosafety Level: 1
Use Restriction: U.S. Pat. 5,290,684
Patent Statement: U.S. Pat. 5,290,684
Reference: U.S. Pat. 5,290,684 dated Mar. 1, 1994
Shipping/Field/Price:
Frozen Vial for all NTD$ 6000 Order this item

Bioresource Collection and Research Center

331, Shih-Pin Rd., Hsinchu 300193, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

Copyright © 2018.BCRC All rights reserved.

web maintainance: +886-3-5223191 ext 572