Cell Line



BCRC Number: 60173 Add To Bookmark
Name: BW5147.G.1.4.OUAR.1
Type: Mouse AKR/J T cell lymphoma
Species: Mus musculus (mouse)
Strain Ethnicity: AKR/J
Gender: unknown
Tissue: lymphoma; T lymphocyte

Morphology: Lymphoblast
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;horse serum,10%.
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 1-4 x 104 cells/ml; a saturation density of 1-2 x 105 cells/ml is obtainable
Medium Renewal: every 2 to 3 days

Description: The cells are ouabain resistant; they can be used to create T cell hybridomas.

Image / Description:   200倍視野下
  100倍視野下
 

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 52: 429-436, 1974
Shipping/Field/Price:
Frozen Vial for all NTD$ 7000 Order this item

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