Cell Line

BCRC Number: 60164 Add To Bookmark
Name: GBM 8901
Type: Human brain glioblastoma multiforme
Species: Homo sapiens (human)
Strain Ethnicity: Asian (Chinese)
Gender: Female
Tissue: brain; glioblastoma; mutilforme

Split Ratio: 1:8 to 1:10
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative; NOTE: The line has been cured of a previous mycoplasma infection at BCRC.
Tumorigenic: yes

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: RPMI 1640 medium,90%;fetal bovine serum,10%.
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Deposit History: Obtained from Dr. Wei-Hwa Lee, Tri-service General Hospital, Taipei, Taiwan.
Description: Established from brain GBM tumor of a Chinese patient; it is tumorigenic in the athymic nude mice with multiple pulmonary metastases; scanning electron microscope examinations revealed the numerous surface microvilli and blebs of various size on the primary GBM tumors, the cultured tumor cells in vitro and the xenografted tumor cells in the nude mice in vivo; the distinct ultrastructural features of stellate cultured cells with astrocytic-differentiation after db-cAMP treatment of 96 hours showed many intermittent dilations (pseudopods) along the long cytoplasmic and the growth cones also expressed strongly positive immunostaining for GFAP; the growth inhibition of db-cAMp treated, cultured in vitro tumor cells was also observed; it may be useful for the investigation of human brain GBM. BCRC confirmed BCRC 60164 GBM8901 was derived from the same donor as BCRC 60163 GBM8401 by using STR-PCR method.
Others: STR-PCR profile — D7S820:11, 12 CSF1PO:9, 10 TH01:6, 7 D13S317:8, 9 D16S539:12, 13 vWA:17, 18 TPOX:8, 11 Amelogenin:X D5S818:11, 13

Image / Description:   200倍視野下(high density)
  200倍視野下(low density)

Biosafety Level: 1
Use Restriction: Need the approval from the depositor (Dr. Wei-Hwa Lee) for distribution
Reference: Chin. Med. J. (Taipei) 48: 177-184, 1991
Frozen Vial for all NTD$ 6000 Order this item

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