Cell Line



BCRC Number: 60161
Name: A-431
Type: Human epidermoid carcinoma
Species: Homo sapiens (human)
Age Stage: 85 years
Gender: Female
Tissue: Epidermis; epidermoid carcinoma

Morphology: Epithelial
Split Ratio: 1:3 to 1:8
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Cytogenetics: hypertriploid
Karyology: Hypertriploid; modal number = 74
Virus susceptibility: yes, forms rapidly growing subcutaneous tumors in immunosuppressed mice and colonies in soft agar

Incubation Temp(°C): 37°C
Culture Medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine, 1 mM sodium pyruvate, adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose,90%;fetal bovine serum,10%.
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: The epidermoid carcinoma cell line A-431, derived from an 85-year-old female, is one of a series of cell lines established from solid tumors by D.J. Giard, et al.
Others: STR-PCR profile — D7S820:10 CSF1PO:11, 12 TH01:9 D13S317:9, 13 D16S539:12, 14 vWA:15, 17 TPOX:11 Amelogenin:X D5S818:12, 13

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 51:1417-1423, 1973
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