Cell Line

BCRC Number: 60157 Add To Bookmark
Name: WiDr
Type: Human colon adenocarcinoma
Species: Homo sapiens (human)
Age Stage: 78 years
Gender: Female
Tissue: colon; colorectal adenocarcinoma

Morphology: Epithelial
Split Ratio: 1:10 to 1:20
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Karyology: Hypertrioploid; modal number = 72
Tumorigenic: yes,in nude mice (tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10{7} cells)
Receptor expressed: epidermal growth factor (EGF)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate,90%;fetal bovine serum,10%.
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Although WiDr deposited as a colon adenocarcinoma line established from a 78 year old female, DNA fingerprinting has shown this line to be a derivative of HT-29; the cells are negative for Colon Antigen 3 expression; the cells are positive for keratin by immunoperoxidase staining; the cells expressed p53 antigen (the p53 produced has a G -> A mutation resulting in Arg -> His at position 273); growth of WiDr cells is inhibited by tumor necrosis factor alpha (TNF alpha); inhibitors of dihydrofolate reductase are highly cytotoxic to WiDr cells.
Others: STR-PCR profile — D7S820:10 CSF1PO:11, 12 TH01:6, 9 D13S317:11 D16S539:11, 12 vWA:17, 19 TPOX:8, 9 Amelogenin:X D5S818:11, 12
Product Character: carcinoembryonic antigen (CEA) 118 ng/106 cells/10 days; Colon Specific Antigen (CSAp); transforming growth factor beta; keratin

Image / Description:   100倍視野下

Biosafety Level: 1
Reference: In Vitro 15: 401-408, 1979: Cancer Res. 34: 105-108, 1974; Science 199: 980-983, 1978; ibid., 230: 943-945, 1985; Cancer Genet. Cytogenet. 27: 125-134, 1987; Cancer Res. 38: 3745-3750, 1978
Frozen Vial for all NTD$ 6000 Order this item

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