Cell Line



BCRC Number: 60149 Add To Bookmark
Name: T84
Type: Human colon carcinoma
Species: Homo sapiens (human)
Age Stage: 72 years
Gender: Male
Tissue: Colon; carcinoma; from metastatic site: lung

Morphology: Epithelial
Split Ratio: 1:2 to 1:4
Isoenzyme Analysis: NP; G6PD, B; MD; LD
Mycoplasma Test: Negative
Karyology: Hyperdiploid; modal number = 56
Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
Receptor expressed: peptide hormone; neurotransmitter

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: A 1:1 mixture of Ham's F-12 medium and DMEM with 2.5 mM L-glutamine,95%; fetal bovine serum,5%.
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: This is a transplantable human carcinoma cell line derived from a lung metastasis of a colon carcinoma in a 72-year-old male; tumor tissue was inoculated subcutaneously and serially transplanted in BALB/c nude mice; the original histological characteristics of the colon carcinoma were maintained throughout transplantation in nude mice; the cells have receptors for many peptide hormones and neurotransmitters and maintain vectorial electrolyte transport; this line exhibits tight junctions, and desmosomes between adjacent cells; the cells should be maintained at high density (at least 1/4 confluency); the cells are positive for keratin by immunoperoxidase staining.
Others: STR-PCR profile — D7S820:8, 10 CSF1PO:10 TH01:6, 9 D13S317:9 D16S539:10, 11 vWA:17, 18 TPOX:8, 11 Amelogenin:X D5S818:12
Product Character: carcinoembryonic antigen (CEA), 600 ng/ml per 106 cells per 10 days; keratin

Image / Description:   200倍視野下
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Biosafety Level: 1
Reference: Proc. Natl. Acad. Sci. USA 77: 3464-3468, 1980; Am. J. Physiol. 246: 204-208, 1984
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