Cell Line



BCRC Number: 60148 Add To Bookmark
Name: LoVo
Type: Human colon adenocarcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 56 years
Gender: Male
Tissue: colon; colorectal adenocarcinoma; metastatic site: left supraclavicular region

Morphology: Epithelial
Split Ratio: 1:2 to 1:10
Isoenzyme Analysis: NP; G6PD, B; MD; LD
Mycoplasma Test: Negative; NOTE: The line has been cured of a previous mycoplasma infection at ATCC.
Karyology: Hyperdiploid

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: Ham's F-12 medium, 80%;fetal bovine serum,20%.
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: The line was initiated from a fragment of a metastatic tumor nodule in the left supraclavicular region of a 56-year-old Caucasian male patient with a histologically proven diagnosis of adenocarcinoma of the colon; the cells are negative for expression of CSAp (CSAp-) and colon antigen 3; the line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes; N-myc and sis oncogene expression were not detected; tumor specific nuclear matrix proteins CC-3 and CC-4 are expressed.
Others: STR-PCR profile — D7S820:10, 11 CSF1PO:11, 13, 14 TH01:9.3 D13S317:8, 11 D16S539:9, 12 vWA:17, 18 TPOX:8, 9 Amelogenin:X, Y D5S818:11, 12, 13
Product Character: carcinoembryonic antigen (CEA) 908 ng/106 cells/10 days

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Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 61: 75-83, 1978; Exp. Cell Res. 101: 414-416, 1976; Cancer Res. 36: 467-475, 1976; Cancer Res. 39: 2630-2636, 1979; Int. J. Cancer 41: 287-296, 1988; Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994
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