Cell Line

BCRC Number: 60140 Add To Bookmark
Name: J774A.1
Type: Mouse macrophage
Species: Mus musculus (mouse)
Strain Ethnicity: BALB/c
Gender: Female
Tissue: ascites; monocyte/macrophage; reticulum cell sarcoma

Morphology: Macrophage
Split Ratio: 1:3 to 1:6
Mycoplasma Test: Negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose,90%;fetal bovine serum,10%.
Growth Property: Adherent
SubCulture Procedure: by scraping: 使用cell scraper 將細胞刮下,再以新鮮培養基稀釋,再分裝至新的培養瓶。或是離心後更換培養基。
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: The line was adapted to culture from a tumor which arose in a female BALB/c mouse; its growth is inhibited by dextran sulfate, PPD and LPS; the line will synthesize large amounts of lysozyme and exhibit minor cytolysis but predominantly antibody-dependent phagocytosis.
Product Character: interleukin-1 (interleukin 1, IL-1, LAF); lysozyme

Image / Description:   100倍視野下

Biosafety Level: 1
Reference: J. Exp. Med. 143: 1528-1533, 1976; J. Immunol. 119: 950-954, 1977; Cancer Res. 37: 546-550, 1977; J. Immunol. 114: 898-905, 1975; Nature 257: 393-394, 1975
Frozen Vial for all NTD$ 6000 Order this item

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