Cell Line



BCRC Number: 60139
Name: MIA PaCa-2
Type: Human pancreatic carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 65 years
Gender: Male
Tissue: Pancreas; carcinoma

Morphology: Epithelial
Split Ratio: 1:3 to 1:8
Isoenzyme Analysis: NP; G6PD, B; MD; LD
Mycoplasma Test: Negative
Cytogenetics: hypotriploid human cell line; modal chromosome number is 61
Karyology: Hypotriploid; modal number = 61

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 87.5% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum + 2.5% horse serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: established from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male; the line is sensitive to asparaginase
Others: STR-PCR profile — D7S820:12, 13 CSF1PO:10 TH01:9, 10 D13S317:12, 13 D16S539:10, 13 vWA:15 TPOX:9 Amelogenin:X D5S818:12, 13
Product Character: human colony stimulating factor, subclass I (CSF-I); plasminogen activator

Image / Description:   200倍視野下
  100倍視野下
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Biosafety Level: 1
Reference: Int. J. Cancer 19:128-135, 1977; Biochemistry 16: 1908-1913, 1977
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