Cell Line



BCRC Number: 60138
Name: A9
Type: Mouse connective tissue, APRT and HPRT deficient, derivative of Strain L cells
Species: Mus musculus (mouse)
Strain Ethnicity: C3H/An
Age Stage: 100 days
Gender: Male
Tissue: subcutaneous connective tissue; normal; areolar and adipose

Morphology: Epithelial
Split Ratio: 1:4 to 1:10
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Karyology: Hyperdiploid; modal number = 52
Tumorigenic: yes, Tumors developed within 2 weekss at 100% frequency (5/5) in nude mice inoculated subcutaneously with 3.5x10(7) cells

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4.5g/L glucose,90% + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: isolated from wild-type Strain L cells; the cells are sensitive to HAT selection medium and deficient in adenosine phosphoribosyl transferase (APRT) and hypoxanthine phosphoribosyl transferase (HPRT); the cells are resistant to 8-azaguanine (0.003 mg/ml) and 6-thioguanine (100mcg)

Biosafety Level: 1
Reference: Proc. Natl. Acad. Sci. USA 50:568-576, 1963
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