Cell Line



BCRC Number: 60137
Name: CCRF-CEM
Type: Human acute lymphoblastic leukemia, T cell
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 4 years
Gender: Female
Tissue: leukemia; acute lymphoblastic; T lymphoblast

Morphology: Lymphoblast
Split Ratio: maintain cell density between 105 to 106 cells/ml
Isoenzyme Analysis: NP; G6PD, B; MD; LD
Mycoplasma Test: Negative
Tumorigenic: yes, in syrian hamsters

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 80% RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate+20% fetal bovine serum
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 2-3 x105 cells/ml
Medium Renewal: every 2 to 3 days

Description: this is a T lymphoblastoid cell line derived from peripheral blood buffy coat of a 4-year-old Caucasian female with acute lymphoblastic leukemia
Others: STR-PCR profile — D7S820:9, 13 CSF1PO:10, 11 TH01:6, 7 D13S317:11, 12 D16S539:10, 13 vWA:17, 19 TPOX:8 Amelogenin:X D5S818:12, 13

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: Cancer 18: 522-529, 1965; Cancer 19: 1725-1742, 1966;Exp. Cell Res. 40:197-200,1965. Cancer Res. 27: 772-783, 1967
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